Medium Containing Trypan Blue and Antibiotics for the Detection of Cryptococcus neoformans in Clinical Samples
نویسندگان
چکیده
منابع مشابه
DETECTION OF CRYPTOCOCCUS NEOFORMANS BY SEMINESTED PCR IN CEREBROSPINAL FLUID
ABSTRACT Life-threatening infections caused by the encapsulated fungal pathogen Cryptococcus neoformans have been increasing steadily over the past 10 years. Cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (CNS) in immunocompetent as well as immunocompromised patients. We report the development of a semi-nested- PCR-based assay for the ...
متن کاملPrimary isolation medium for Cryptococcus neoformans.
The isolation and identification of Cryptococcus neoformans is improved by use of a potato dextrose medium containing urea-antibiotic supplements and a pH of 3.5.
متن کاملdetection of cryptococcus neoformans by seminested pcr in cerebrospinal fluid
abstract life-threatening infections caused by the encapsulated fungal pathogen cryptococcus neoformans have been increasing steadily over the past 10 years. cryptococcus neoformans is recognized as the most frequent fungal infection of the central nervous system (cns) in immunocompetent as well as immunocompromised patients. we report the development of a semi-nested- pcr-based assay for the d...
متن کاملEsculin-based medium for isolation and identification of Cryptococcus neoformans.
A simple medium was developed, using esculin as the substrate, for the isolation and identification of Cryptococcus neoformans. C. neoformans produced a brown-black pigment on the medium; all other yeasts produced no pigment or were light yellow. Esculin is beta-glucose-6,7-dihydroxycoumarin. C. neoformans produced pigment because the 6,7-dihydroxycoumarin component of the esculin molecule was ...
متن کاملapplication and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples
ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.27.1.38-42.1974